Inclusion Body

In our embryo lab, we often seen eggs with an inclusion body. Especially with patients' who had an endometriosis, pelvic inflammatory disease (PID), hydrosalphinx and polycyctic ovarian syndrome (PCOS). It always interrupting the fertilization of the egg. When we seen eggs with inclusion body, we already knew, that particular egg might not be fertilize. So, i did some research about what is actually "inclusion body" and how does it form. Here are some information about inclusion body that i manage to get from the Internet. 

According to Matt Bird in his article "Types and causes of inclusion bodies in human cells", inclusion body is an abnormal structural that appears in the cells. They're typically associated with proteins, and are sure signs of the presence of a virus or, in some extreme cases, genetic diseases. They are foreign invaders, and unwelcome in any living body. Inclusion bodies are typically identified within a cell both by their appearance under a microscope and, by connection, the practice of staining. How inclusion cells get into human cells varies from virus to virus and disease to disease, and in many cases it's yet unknown - or concretely known, anyway - how a person can wind up with inclusion bodies in their cells, and thus suffer the resulting consequences.

The nature of inclusion bodies is somewhat hinted at in the name: they're abnormal structures that build up in the nucleus or cytoplasm of cells, both human and animal. These structures, usually visible after staining and observation under a microscope, heavily hint at the presence of foul play in a cell, whether viral or genetic. Usually composed of protein, inclusion bodies are not a pleasant find and usually indicate current trouble or problems to come in a body. No wonder, patients' with an inclusion body would not fertilize.

According to Wikipedia, protein inclusion bodies are classically thought to contain misfolded protein. However, this has recently been contested, as green fluorescent protein will sometimes fluoresce in inclusion bodies, which indicates some resemblance of the native structure and researchers have recovered folded protein from inclusion bodies.